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Frank P. Patients should be mobilized within 24 to 48 hours if possible to prevent complications of immobility. Godin, B. Simon, 466 p150Glued, 638 Phalloidin fluorescently labeled, 635 -stabilized F-actin, preparation of, 700 Phase-contrast microscopy, 634, 649 Phenylmethylsulfonyl fluoride (PMSF), an inhibitor of serine proteases, 7 Pheochromocytoma, causes for, 213 Phosphates, high-energy, in animal tissue and cultured cells, measurement by HPLC, 165169 Phosphocreatine, measurement of, 168169 Phosphoimager system, 470 Phosphorylated nucleotides, measurement of, 168169 Phosphorylation complexes, oxidative, supramolecular assemblies of, 736 Photobleaching, 633 of GFPDsRed targeted to mitochondria, 634 and phototoxicity, 648649 Phototoxicity, indicators of, 648 Phytohemagglutinin, 519 Picopure DNA extraction kit, 487 Plan NeoFluarÒ oil immersion objectives, 643 Plant mitochondria from Arabidopsis thaliana cell culture, 72 assays function, 7577 enzyme activities, assessment of, 76 growth and preparation of plant material for, 6667 integrity determinations, 7475 isolation and subfractionation from, 6687 isolation of, 6769 homogenization, 6768 pH of medium following homogenization, 6768 preparation of medium and plant material for, 6768 ratio of homogenization medium to plant tissue, 67 in organello translation analysis, 8384 preparations for screening transgenic lines, 7172 purification of, 6973 cell callus mitochondria preparation, 7073 percoll gradient for, 6972 preparations for screening transgenic lines, 7172 sucrose gradient for, 6970 purification of enzymes from, 76 purity determinations, 74 storage, 75 subfractionation compartment, 7783 yield calculations, 7374 Plasma carnitine and acylcarnitine, protocol for measurement of, 183184 Plasma membrane carnitine transporter, 181 Plasmid constructs, use of, 786 Plasmid DNA, 488 Plasmids, use in in vitro fusion assays, 709710 Platelets, as mtDNA donors, 512 Point mutations, 530 in mtDNA, 449451, 574 detection by PCRRFLP analysis, 451456 quantification of heteroplasmy level by ARMS and quantitative Real-Time PCR, 456459 Poissonian probability, 498 Polarography, 559 Polymerase chain reaction (PCR), 786, see also Real-Time PCR competitive, quantition of mtDNA by, 470472 jumping, 498 long-range, 496 nested increasing amplification effciency using, 488489 product detection sensitivity, enhancing using radioactive labeling, 488 RFLP of DNA, 492 signal strength, from microdissected mtDNA samples, strategies for improving, 487489 typing, 515 Polymorphisms in mtDNA populations, 489 and mutations (mtDNA), RFLP of, 489490 Polyvinylidene difluoride (PVDF), 775 Porcine adrenal cortex, mitochondria from, 3031 Potter-Elvehjem homogenizers, 5 Potter-Elvejhem homogenizer, 9, 16 Precipitation reactions, 555 Presequences mitochondrial, potential of, 764 by MPP, processing of, 765766 on surface of mitochondria, interactions of, 765 Figure 1.first Forex Brokers Wustrow such the
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